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rage x Oi / 




OIPE 



RAW SEQUENCE LISTING 
PATENT APPLICATION: US/09/981, 124 

Input Set : A:\es.txt 

Output Set: N:\CRF3\11092001\I981124.raw 



DATE: 11/09/2001 
TIME: 10:23:22 



APPLICANT: Green, Allan 
Singh, Surinder 
Lenman, Marit 
Stymne, Sten 

OF INVENTION: FATTY 



ACID EPOXYGENASE GENES FROM PLANTS AND USES THEREFOR 



f 



3 <110> 
4 
5 
6 

8 <120> TITLE 
IN MODIFYING 

9 FATTY ACID METABOLISM 
11 <130> FILE REFERENCE: 26-98A 

C--> 13 <140> CURRENT APPLICATION NUMBER: US/09/981, 124 *\ 
C--> 13 <141> CURRENT FILING DATE: 2001-10-17 ^ 

13 <150> PRIOR APPLICATION NUMBER: US 09/059769 

14 <151> PRIOR FILING DATE: 1998-04-14 

16 <150> PRIOR APPLICATION NUMBER: US 60/043706 

17 <151> PRIOR FILING DATE: 1997-04-16 

19 <150> PRIOR APPLICATION NUMBER: AU P06223 

20 <151> PRIOR FILING DATE: 1997-04-15 

22 <150> PRIOR APPLICATION NUMBER: AU P06226 

23 <151> PRIOR FILING DATE: 1997-04-15 

25 <150> PRIOR APPLICATION NUMBER: US 60/050403 

26 <151> PRIOR FILING DATE: 1997-06-20 
28 <160> NUMBER OF SEQ ID NOS : 24 

30 <170> SOFTWARE: Patentln version 3.1 

32 <210> SEQ ID NO: 1 

33 <211> LENGTH: 1358 

34 <212> TYPE: DNA 

35 <213> ORGANISM: Crepis palaestina 

37 <220> FEATURE: 

38 <221> NAME/KEY: CDS 

39 <222> LOCATION: (30).. (1151) 

40 <223> OTHER INFORMATION: 

43 <400> SEQUENCE: 1 

44 gagaagttga ccataaatca tttatcaac atg ggt gcc ggc ggt cgt ggt egg 

45 Met Gly Ala Gly Gly Arg Gly Arg 

46 15 



ENTERED 




53 



48 


aca 


teg 


gaa 


aaa 


teg 


gtc 


atg 


gaa 


cgt 


gtc 


tea 


gtt 


gat 


cea 


gta 


acc 


101 


49 


Thr 


Ser 


Glu 


Lys 


Ser 


Val 


Met 


Glu 


Arg 


Val 


Ser 


Val 


Asp 


Pro 


Val 


Thr 




50 




10 










15 










20 












52 


ttc 


tea 


ctg 


agt 


gaa 


ttg 


aag 


caa 


gca 


ate 


cct 


ccc 


cat 


tgc 


ttc 


cag 


149 


53 


Phe 


Ser 


Leu 


Ser 


Glu 


Leu 


Lys 


Gin 


Ala 


He 


Pro 


Pro 


His 


Cys 


Phe 


Gin 




54 


25 










30 










35 










40 




56 


aga 


tct 


gta 


ate 


cgc 


tea 


tct 


tac 


tat 


gtt 


gtt 


caa 


gat 


etc 


att 


att 


197 


57 


Arg 


Ser 


Val 


He 


Arg 


Ser 


Ser 


Tyr 


Tyr 


Val 


Val 


Gin 


Asp 


Leu 


He 


He 




58 










45 










50 










55 






60 


gcc 


tac 


ate 


ttc 


tac 


ttc 


ctt 


gcc 


aac 


aca 


tat 


ate 


cct 


act 


ctt 


cct 


245 


61 


Ala 


Tyr 


He 


Phe 


Tyr 


Phe 


Leu 


Ala 


Asn 


Thr 


Tyr 


He 


Pro 


Thr 


Leu 


Pro 




62 








60 










65 










70 








64 


act 


agt 


eta 


gcc 


tac 


tta 


get 


tgg 


ccc 


gtt 


tac 


tgg 


ttc 


tgt 


caa 


get 


293 


65 


Thr 


Ser 


Leu 


Ala 


Tyr 


Leu 


Ala 


Trp 


Pro 


Val 


Tyr 


Trp 


Phe 


Cys 


Gin 


Ala 





file://C:\Crf3\Outhold\VsrI98 1 1 24.htm 



11/9/01 



rage l or / 

RAW SEQUENCE LISTING DATE: 11/09/2001 

PATENT APPLICATION: US/09/981, 124 TIME: 10:23:22 



Input Set :. A:\es.txt 

Output Set: N:\CRF3\11092001\I981124.raw 







75 










80 










85 










o O ciy u 


rr t* n 

y uu 


pt p 


a pt 


doc 


tta 


L-y y 


atp 


etc 


y y 


cac 


aaa 

V4 *w4i 


tcrt 


crcrt 


cac 


cat 


341 


(Z Q Coy* 
O Dei. 


Va 1 

V ul 


T.pn 

ueix 


Thr 

X 11X 


\j j. y 






He 




Glv 


His 


Glu 


Cvs 


Glv 


His 


His 




70 


90 










95 










100 












7 9 rrpp 


ttt 

U L* L- 


a ac 
a y o 


a a p 


tap 




<-y y 


ttt 

L- ^> 




crac 

y W4 V* 


act 


crtcr 
y *-y 


crcrc 


ttc 


ate 


etc 


389 


/ J Ala 


Pho 


Cot* 

OCX 


noil 


iyr 


J. 11X 




c lie 


n o w 


A QD 


Thr 


Val 


Glv 

vjj.y 


Phe 

JJL JJk 1 


He 


Leu 




7 4 in^ 










110 

-i- -1. V 










115 










120 




7 £ r«a r" 


+- a 

UUd 


ttt 

U U U 


P t P 
U 


pt r* 


a pp 


ppct 


fat 


ttP 


tct 


uy y 


aaa 


ttc 


acrt 


cac 


cac 
y 


437 


77 U*i a. 
/ / nib 


Del. 


Pho 
rile 


T.on 

1JCU 




± 11X 


P"TPi 


X jf X 


Phe 

t ixe 


e ju 


XX p 


u y o 


Phe 


Ser 


His 


Ara 




7 fl 








1 9S 

X Z J 










130 

-l- \s 










135 

v v 






OA a a 4- 

0 U aaL 


Oat 


Ld L 


t" ft r** 
UU-U 


a a r* 


a pa 


a <t t - 
ay L- 


f PfT 

uuy 


att 
a i_ l. 


rra t 
yen. 


a a p 
aa^> 


fiat 

y a (— 


era a 


att 
y u u 


tap 


att 


485 


PI Son 
OX noil 


n x £■> 


Ui* a 
tils 


Cor 


R on 
noil 


J. 11X 


Cot* 
oex 


Cot* 


Hp 

ju jl e 


A Qn 

n o^ 


A ciri 
no ii 


A 

no 


Glu 

\J -L. U 


Val 


Tvr 

x _y x 


He 




o z 






X *x \J 










145 

JL 1 










150 

w 








04 CCy 


a a a 

add 


dy u 


day 


■too 


a a a 
ddd 


pf p 


guy 


prrt 
y l. 


a t p 
a l.u 


tat 

i^a l. 


aaa 


ctt 

1— I— 


ctt 

^> u> 


a a p 


aap 

c& u 


533 


oo Fro 


i*yo 


Cor 
OCl 


Uj o 


oeX 


Xiy o 


T.on 


aia 

nxa 


A T-pr 

nx y 


Tip 
x xe 


T VT 


xiy & 


XJC Li. 


TiPll 


A 

no ii 


A *?n 

no ii 




o o 




X J 










160 










165 

\J w 










Q Q nn a 

0 0 CCo 


/"i /"i 


ggr. 


f-\ rr /-t 

egg 


ptrr 

t_y 


-t -t rr 

l. uy 


rrr t 


-tta 

i-i-g 


aft 


a t p 
a 


a t rr 
a uy 


ttP 


a pp 

a o o 


pt 1 a 


aaa 

y y a 


ttt 

L» K- 


581 

\J _JU 


Q Q n r « 
0 j rlO 


D Y*r*t 
IT X \J 


biy 


rVX y 


T iOi i 


JJCU 


Va 1 
v a x 


xie ix 


Tip 
x x e 


Tip 


Mpt 
lie v. 


Phe 

f 1 1 


Thr 


Leu 


GlV 


Phe 

JL Jl A 






1 70 
X / v 










175 










180 

V V 












.7 Z U 


tta 
L. La 


UdU 




l, uy 


a pfl 


aa l. 


att 


t PP 


y y u- 


a acr 

aay 


aaa 


tac 


aac 

*j \^ 


aaa 
y y 


ttt 


629 


Q "3 Dm 
.7 O JrXtJ 


lieu 


Tvt* 

lyx 


LCU 




■L 11J- 


A QT1 
no ii 


He 

x J. e 


Ser 


Glv 


T.VS 


LVS 

XJ J o 


Tvr 

X X 


ASD 


Ara 


Phe 

Jb Jl JV 




Q4 1 fi^ 










190 










195 










200 




QC nor** 
jv) y 


a a p 

d d U 


r*a r* 






ppp 


a uy 


a cr1" 

U M L. 


ppa 


att 


ttc 


aaa 


\A Ia wl 


cat 


aaa 

y °-y 


caa 
^y y 


677 


Q7 Ala 
7 / nXd 


Aon 


U*i Q 
ni o 


Ir xie 


A en 
rio^ 


IT I V 


lie l. 




Prn 


He 


Phe 

C 11C 


xiy o 


Glu 


Ara 

nx y 


Glu 

«1» 1A 


Ara 




-7 O 








205 










210 










215 






i nn ttt 

1UU U U U 




y lc 


ftp 


n+*t" 


t" Pft 

L.L^y 


nra t" 
y a l. 


Ptt 
U L. L. 


y y l- 


Ptt 


ptt 


(JPP 

M O 


y *-y 


ttt 

V— fc— 


tat 


aaa 
y y c * 


725 


-LUX irlle 




Va 1 

V d X 


xr lie 


T.on 
xie u. 


Cot 
oex 


no 


JJCU 


vjj. y 




xjc li 


Ala 

£i _L u 


Val 


Phe 


Tvr 


Glv 




1U z 






220 

At Z VJ 










225 

Mmi 










230 








1 HA aft 

1U<± all 


a o a 

ddd 


rrt t 
y u u 


y u 


rr*t a 
y Ld 


y oa 


aat 

aa L, 


a a a 
aaa 


y y a 


CTPt 
y u l. 


CTPt" 

M ^ L> 


t crcr 
uy y 


cr"t"a 


aca 

y uy 


tac 


ata 


773 


1UD 116 


Tun 

■Lys 


\7a 1 

Val 


a 1 a 
Hid 


v ai 


a 1 a 
Aid 


noil 


T AT o 

juy o 


o xy 


Al a 
nlu 


Ala 

nXd 


xxp 


Va 1 

V d X 


Al a 

nxa 


uy o 


Met 

1*1 C L- 




1UD 




9 1 S 
^ j j 










240 

z *± V 










245 










i no = 

lUo XLaX. 


g aa 




ccg 


i^r +- a 

y i-d 


Ltd 


fr rt r* 

gge 


pr+- a 

y Ld 


ttt 

L.LL 


a /**p 


ttt 
u u u 


tt P 
u uu 


era t 
y a l. 


rrt a 
y Ly 


a t P 
a L- ^ — 


a pp 


821 

Ua.1 


iuy ±yr 


P 1 -ir 

uiy 


va 1 

V al 


Jr X \J 


Va 1 

v dx 


T oil 

XteU, 


Pi v 
Vjxy 


Va 1 

V d X 


Pbo 
i lie 


TV» t 

X 11X 


Php 
Jr lie 


Php 

Jr lie 


A Qn 


Va 1 

v ax 


He 

J. -L 


Th r 

X ill 




11U 












9 I S5 

£t *J -J 










260 

A V V 












110 ttr< 
11Z UL.U 


-t 4- rr 

uuy 


nap 

Cdu. 




a o o 




pa/t 

L.uy 


u^y 


t Prf 
u^y 


ppt 
k. 


pa t 


tat 


era t 
y a 


t pa 

L.*w>a 


a pt 


aa a 

VJ s^L KJL 


869 

SJ \J J 


11J irixe 




nib 


Un e 
nib 


T Vi y 
1 111. 


Ui a 
fix o 




Cot 

SCI 


Cot 

OCX 


Prn 

Jr X \J 


U-i g 
XI X O 


Tvt 
iyx 


A QTi 
no iy 


Cor 

tj ex 


Thr 


Glu 

VJJ J. Ll 




111 Z D J 










270 










275 










280 




1 1 t rrn 
j. _l d uy y 


a a /-< 


t rrrr 

uyy 


a. 


a rra 
ay a 


yyy 


rrpp 

y \-f \*r 


tta 


t pa 


ana 


at p 


era f 


acrcr 

ay y 


aac 


ttt 

W <v> •w* 


aaa 
y y *-* 


917 


11/ Trp 


Asn 


Trp 


lie 


Arg 


(jiy 


Ala 

Aia 


T" * *i 

lieu 


O /~\ >^ 


Ala 

Aia 


Tl o 
lie 


ASp 


nx y 


a c Tt 

nop 


Pho 


P-1 V 

oxy 




118 








285 










290 










295 






120 ttc 


ctg 


aat 


agt 


gtt 


ttc 


cat 


gat 


gtt 


aca 


cac 


act 


cat 


gtc 


atg 


cat 


965 


121 Phe 


Leu 


Asn 


Ser 


Val 


Phe 


His 


Asp 


Val 


Thr 


His 


Thr 


His 


Val 


Met 


His 




122 






300 










305 










310 








124 cat 


ttg 


ttt 


tea 


tac 


att 


cca 


cac 


tat 


cat 


gca 


aag 


gag 


gca 


agg 


gat 


1013 


125 His 


Leu 


Phe 


Ser 


Tyr 


He 


Pro 


His 


Tyr 


His 


Ala 


Lys 


Glu 


Ala 


Arg 


Asp 




126 




315 










320 










325 










128 gca 


ate 


aag 


cca 


ate 


ttg 


ggc 


gac 


ttt 


tat 


atg 


ate 


gac 


agg 


act 


cca 


1061 


129 Ala 


He 


Lys 


Pro 


He 


Leu 


Gly 


Asp 


Phe 


Tyr 


Met 


He 


Asp 


Arg 


Thr 


Pro 




130 


330 










335 










340 













file://C:\Crf3\Outhold\VsrI98 1 1 24.htm 



11/9/01 



rage D ui / 



RAW SEQUENCE LISTING DATE: 11/09/2001 

PATENT APPLICATION: US/09/981, 124 TIME: 10:23:22 

Input Set : A:\es.txt 

Output Set: N:\CRF3\11092001\I981124.raw 

132 att tta aaa gca atg tgg aga gag ggc agg gag tgc atg tac ate gag 1109 

133 He Leu Lys Ala Met Trp Arg Glu Gly Arg Glu Cys Met Tyr He Glu 

134 345 350 355 360 

136 cct gat age aag etc aaa ggt gtt tat tgg tat cat aaa ttg 1151 

137 Pro Asp Ser Lys Leu Lys Gly Val Tyr Trp Tyr His Lys Leu 

138 365 370 

140 tgatcatatg caaaatgeae atgeatttte aaaccctcta gttacgtttg ttctatgtat 1211 
142 aataaacege eggtcctttg gttgactatg cctaagccag gcgaaacagt taaataatat 1271 
144 eggtatgatg tgtaatgaaa gtatgtggtt gtctggtttt gttgctatga aagaaagtat 1331 
146 gtggttgtcg gtcaaaaaaa aaaaaaa 1358 

149 <210> SEQ ID NO: 2 

150 <211> LENGTH: 374 

151 <212> TYPE: PRT 

152 <213> ORGANISM: Crepis palaestina 
154 <400> SEQUENCE: 2 

156 Met Gly Ala Gly Gly Arg Gly Arg Thr Ser Glu Lys Ser Val Met Glu 

157 15 10 15 

160 Arg Val Ser Val Asp Pro Val Thr Phe Ser Leu Ser Glu Leu Lys Gin 

161 20 25 30 

164 Ala He Pro Pro His Cys Phe Gin Arg Ser Val He Arg Ser Ser Tyr 

165 35 40 45 

168 Tyr Val Val Gin Asp Leu He He Ala Tyr He Phe Tyr Phe Leu Ala 

169 50 55 60 

172 Asn Thr Tyr He Pro Thr Leu Pro Thr Ser Leu Ala Tyr Leu Ala Trp 

173 65 70 75 80 

176 Pro Val Tyr Trp Phe Cys Gin Ala Ser Val Leu Thr Gly Leu Trp He 

177 85 90 95 

180 Leu Gly His Glu Cys Gly His His Ala Phe Ser Asn Tyr Thr Trp Phe 

181 100 105 110 

184 Asp Asp Thr Val Gly Phe He Leu His Ser Phe Leu Leu Thr Pro Tyr 

185 115 120 125 

188 Phe Ser Trp Lys Phe Ser His Arg Asn His His Ser Asn Thr Ser Ser 

189 130 135 140 

192 He Asp Asn Asp Glu Val Tyr lie Pro Lys Ser Lys Ser Lys Leu Ala 

193 145 150 155 160 

196 Arg He Tyr Lys Leu Leu Asn Asn Pro Pro Gly Arg Leu Leu Val Leu 

197 165 170 175 

200 He He Met Phe Thr Leu Gly Phe Pro Leu Tyr Leu Leu Thr Asn He 

201 180 185 190 

204 Ser Gly Lys Lys Tyr Asp Arg Phe Ala Asn His Phe Asp Pro Met Ser 

205 195 200 205 

208 Pro He Phe Lys Glu Arg Glu Arg Phe Gin Val Phe Leu Ser Asp Leu 

209 210 215 220 

212 Gly Leu Leu Ala Val Phe Tyr Gly He Lys Val Ala Val Ala Asn Lys 

213 225 230 235 240 

216 Gly Ala Ala Trp Val Ala Cys Met Tyr Gly Val Pro Val Leu Gly Val 

217 245 250 255 

220 Phe Thr Phe Phe Asp Val He Thr Phe Leu His His Thr His Gin Ser 

221 260 265 270 



file://C:\Crf3\Outhold\VsrI981 124.htm 



11/9/01 



i-age hot f 



RAW SEQUENCE LISTING DATE: 11/09/2001 

PATENT APPLICATION: US/09/981,124 TIME: 10:23:22 



Input Set : A:\es.txt 

Output Set: N:\CRP3\11092001\I981124.raw 



224 


Ser 


Pro 


His 


Tyr 


Asp 


Ser 


Thr 


Glu 


Trp 


Asn 


Trp 


He 


Arg 


Gly Ala Leu 


225 






275 










280 










285 






228 


Ser 


Ala 


He 


Asp 


Arg 


Asp 


Phe 


Gly 


Phe 


Leu 


Asn 


Ser 


Val 


Phe 


His Asp 


229 




290 










295 










300 








232 


Val 


Thr 


His 


Thr 


His 


Val 


Met 


His 


His 


Leu 


Phe 


Ser 


Tyr 


He 


Pro His 


233 


305 










310 










315 








320 


236 


Tyr 


His 


Ala 


Lys 


Glu 


Ala 


Arg 


Asp 


Ala 


He 


Lys 


Pro 


He 


Leu 


Gly Asp 


237 










325 










330 










335 


240 


Phe 


Tyr 


Met 


He 


Asp 


Arg 


Thr 


Pro 


He 


Leu 


Lys 


Ala 


Met 


Trp 


Arg Glu 


241 








340 










345 










350 




244 


Gly Arg 


Glu 


Cys 


Met 


Tyr 


He 


Glu 


Pro 


Asp 


Ser 


Lys 


Leu 


Lys 


Gly Val 


245 






355 










360 










365 






248 


Tyr 


Trp 


Tyr 


His 


Lys 


Leu 





















249 370 

252 <210> SEQ ID NO: 3 

253 <211> LENGTH: 1309 

254 <212> TYPE: DNA 

255 <213> ORGANISM: Crepis sp. 

257 <220> FEATURE: 

258 <221> NAME/KEY: misc_feature 

259 <222> LOCATION: (937).. (937) 

260 <223> OTHER INFORMATION: N is any nucleotide residue 

263 <220> FEATURE: 

264 <221> NAME/KEY: CDS 

265 <222> LOCATION: (26).. (1147) 

266 <223> OTHER INFORMATION: 

269 <220> FEATURE: 

270 <221> NAME/KEY: misc_f eature 

271 <222> LOCATION: (901). .(901) 

272 <223> OTHER INFORMATION: N is any nucleotide residue 
275 <400> SEQUENCE: 3 



276 


tgttgaccat . 


aaatcatcta tcaac 


atg 


ggt 


gee 


ggc 


ggc 


cgt 


ggt 


egg 


teg 


52 


277 
















Met 


Gly Ala 


Gly Gly Arg 


Gly Arg 


Ser 




278 
















1 








5 












280 


gaa 


aag 


teg 


gtc 


atg 


gaa 


cgt 


gtc 


tea 


gtt 


gat 


cca 


gta 


ace 


ttc 


tea 


100 


281 


Glu 


Lys 


Ser 


Val 


Met 


Glu 


Arg 


Val 


Ser 


Val 


Asp 


Pro 


Val 


Thr 


Phe 


Ser 




282 


10 










15 










20 










25 




284 


ctg 


agt 


gat 


ttg 


aag 


caa 


gca 


ate 


cct 


cca 


cat 


tgc 


ttc 


cag 


cga 


tct 


148 


285 


Leu 


Ser 


Asp 


Leu 


Lys 


Gin 


Ala 


He 


Pro 


Pro 


His 


Cys 


Phe 


Gin 


Arg 


Ser 




286 










30 










35 










40 






288 


gtc 


ate 


cgt 


tea 


tct 


tat 


tac 


gtt 


gtt 


cag 


gat 


etc 


ata 


att 


gee 


tac 


196 


289 


Val 


He 


Arg 


Ser 


Ser 


Tyr 


Tyr 


Val 


Val 


Gin 


Asp 


Leu 


He 


He 


Ala 


Tyr 




290 








45 










50 










55 








292 


ate 


ttc 


tac 


ttc 


ctt 


gee 


aac 


aca 


tat 


ate 


cct 


aat 


etc 


cct 


cat 


cct 


244 


293 


He 


Phe 


Tyr 


Phe 


Leu 


Ala 


Asn 


Thr 


Tyr 


He 


Pro 


Asn 


Leu 


Pro 


His 


Pro 




294 






60 










65 










70 










296 


eta 


gec 


tac 


tta 


get 


tgg 


ccg 


ctt 


tac 


tgg 


ttc 


tgt 


caa 


get 


age 


gtc 


292 


297 


Leu 


Ala 


Tyr 


Leu 


Ala 


Trp 


Pro 


Leu 


Tyr 


Trp 


Phe 


Cys 


Gin 


Ala 


Ser 


Val 




298 




75 










80 










85 













file://C:\Crf3\Outhold\VsrI98 1 1 24.htm 



11/9/01 




rage dot f 



RAW SEQUENCE LISTING DATE: 11/09/2001 

PATENT APPLICATION: US/09/981, 124 TIME: 10:23:23 

Input Set : A:\es.txt 

Output Set: N:\CRF3\11092001\I981124.raw 

300 etc act ggg tta tgg ate etc ggc cat gaa tgt ggt cac cat gec tat 340 

301 Leu Thr Gly Leu Trp lie Leu Gly His Glu Cys Gly His His Ala Tyr 

302 90 95 100 105 

304 age aac tac aca tgg gtt gac gac act gtg ggc ttc ate ate cat tea 388 

305 Ser Asn Tyr Thr Trp Val Asp Asp Thr Val Gly Phe lie lie His Ser 

306 110 115 120 

308 ttt etc etc ace ccg tat ttc tct tgg aaa tac agt cac egg aat cac 436 

309 Phe Leu Leu Thr Pro Tyr Phe Ser Trp Lys Tyr Ser His Arg Asn His 

310 125 130 135 

312 cat tec aac aca agt teg att gat aac gat gaa gtt tac att ccg aaa 484 

313 His Ser Asn Thr Ser Ser lie Asp Asn Asp Glu Val Tyr lie Pro Lys 

314 140 145 150 

316 age aag tec aaa etc aag cgt ate tat aaa ctt ctt aac aac cca cct 532 

317 Ser Lys Ser Lys Leu Lys Arg lie Tyr Lys Leu Leu Asn Asn Pro Pro 

318 155 160 165 

320 ggt cga ctg ttg gtt ttg gtt ate atg ttc ace eta gga ttt cct tta 580 

321 Gly Arg Leu Leu Val Leu Val lie Met Phe Thr Leu Gly Phe Pro Leu 

322 170 175 180 185 

324 tac etc ttg aca aat att tec ggc aag aaa tac gat agg ttt gec aac 628 

325 Tyr Leu Leu Thr Asn lie Ser Gly Lys Lys Tyr Asp Arg Phe Ala Asn 

326 190 195 200 

328 cac ttc gac ccc atg agt cca att ttc aaa gaa cgt gag egg ttt cag 676 

329 His Phe Asp Pro Met Ser Pro lie Phe Lys Glu Arg Glu Arg Phe Gin 

330 205 210 215 

332 gtc ttc ctt teg gat ctt ggt ctt ctt get gtg ttt tat gga att aaa 724 

333 Val Phe Leu Ser Asp Leu Gly Leu Leu Ala Val Phe Tyr Gly lie Lys 

334 220 225 230 

336 gtt get gta gca aat aaa gga get get tgg gtg gcg tgc atg tat gga 772 

337 Val Ala Val Ala Asn Lys Gly Ala Ala Trp Val Ala Cys Met Tyr Gly 

338 235 240 245 

340 gtt ccg gtg eta ggc gta ttt acc ttt ttc gat gtg ate acg ttc tta 820 

341 Val Pro Val Leu Gly Val Phe Thr Phe Phe Asp Val lie Thr Phe Leu 

342 250 255 260 265 

344 cac cac acc cat cag teg teg cct cat tat gat tea act gaa tgg aac 868 

345 His His Thr His Gin Ser Ser Pro His Tyr Asp Ser Thr Glu Trp Asn 

346 270 275 280 

W--> 348 tgg ate aga ggg get ttg tea gca ate gat agn gac ttt ggg ttc ctg 916 
W--> 349 Trp lie Arg Gly Ala Leu Ser Ala lie Asp Xaa Asp Phe Gly Phe Leu 

350 285 290 295 

W--> 352 aat agt gtt ttc cat gat gtn aca cac act cac gtc atg cat cat ttg 964 

353 Asn Ser Val Phe His Asp Val Thr His Thr His Val Met His His Leu 

354 300 305 310 

356 ttt tea tac att cca cac tat cat gca aag gaa gca agg gat gca ate 1012 

357 Phe Ser Tyr lie Pro His Tyr His Ala Lys Glu Ala Arg Asp Ala lie 

358 315 320 325 

360 aaa ccg ate ttg ggc gac ttt tat atg ate gat agg act cca att tta 1060 

361 Lys Pro lie Leu Gly Asp Phe Tyr Met lie Asp Arg Thr Pro lie Leu 

362 330 335 340 345 

364 aaa gca atg tgg aga gag ggc agg gaa tgc atg tac ate gag cct gat 1108 



file://C:\Crf3\Outhold\VsrI981 124.htm 



Use of n and / or Xaa has been detected in the 
Sequence Listing. Review the Sequence Listing 
to ensure a corresponding explanation is present 
in the <220> to <223> fields of each sequence 
using n or Xaa. 



11/9/01 



rage o or / 



VERIFICATION SUMMARY 

PATENT APPLICATION: US/09/981,124 



DATE: 11/09/2001 
TIME: 10:23:24 



Input Set : A:\es.txt 

Output Set: N:\CRF3\11092001\I981124.raw 



L:13 M:270 C: Current Application Number differs, Replaced Current Application No 

L:13 M:271 C: Current Filing Date differs, Replaced Current Filing Date 

L:348 M:341 W: (46) "n" or "Xaa" used, for SEQ ID# : 3 

L:349 M:341 W: (46) "n" or "Xaa" used, for SEQ ID# : 3 

L:352 M:341 W: (46) "n" or "Xaa" used, for SEQ ID# : 3 

L:473 M:341 W: (46) "n" or "Xaa" used, for SEQ ID# : 4 

L:1561 M:341 W: (46) "n" or "Xaa" used, for SEQ ID#:21 

L:1580 M:341 W: (46) "n" or "Xaa" used, for SEQ ID#:22 

L:1598 M:341 W: (46) "n" or "Xaa" used, for SEQ ID#:23 

L:1616 M:341 W: (46) "n" or "Xaa" used, for SEQ ID#:24 



file://C:\Crf3\Outhold\VsrI981 124.htm 



11/9/01